Creating Virtual Reality World to Experiment on Real Subjects

A new VR system allows scientists to perform precise experiments that investigate how such sensory cues are integrated into the brain.

Animals are placed in carefully calibrated 3-D VR space.

To maintain the relevant viewpoint as the animal moved around in the space, the team carefully calibrated the 3-D shape of the walls and how each patch of wall was illuminated by each projector pixel.

The team tested the virtual worlds on freely moving fruit flies, zebrafish, and mice. The virtual worlds allowed the team to study mice’s aversion to heights and how zebrafish respond to being swarmed by invaders. The results also show that restrained fly preparations are not suitable for studying the role of head movements in flight control.

“FreemoVR clearly gives the experimenter the power to put virtual objects in a fly’s environment, such as a post, which the animal will react to as if they are real.”

Card says that with systems like FreemoVR, researchers could change the visual stimuli a fly experiences in real time according to the animal’s behavior.  This could help dissect how the fly uses visual information to make behavioral choices.

The new system “does a very nice job of implementing freely moving VR,” agrees Albert Lee, also of Janelia, by email. He studies spatial learning in rats and says that the team’s experiment on mice shows the system can effectively simulate 3-D environmental structure from the animal’s point of view.

In the experiment, the team tested how mice respond to seeing different floor depths by having the animals walk on a circular track elevated above a checkerboard surface. Mice can sense height by using the relative movement of objects to determine their distance, so checks of the same size move differently if they are farther away.

The team then created a VR set-up by changing the projection of the squares so that they moved to give the perspective of being at different heights. While in the static set-up, the animals spent equal amounts of time above different areas of the floor, in both the real world and VR system, the mice spent more time above the flooring that wasn’t as far away.

“The animal reacts by showing a real-world-like ‘fear of heights,’” Lee says. Mice have an aversion to heights and spend most of their time over shallow depths in both real-world and virtual-reality conditions, a new study shows.

The application of the VR system in future mouse neurobiology work, particularly within the pharmaceutical industry.

Source: Virtual Reality for Freely Moving Animals | The Scientist Magazine®